Across kingdom biased CYP-mediated metabolism via small-molecule ligands docking on P450 oxidoreductase

Combining single molecule FRET and single turnover studies with cell studies and docking simulations we advance our understanding on the intricate role of conformational dynamics on activity and specificity, and eventually how pathogenic mutations and small molecule ligand interactions control metabolic disorder and biosynthetic pathways.


DeepFRET, a new deep learning-based platform for standardized, automated, and unbiased single-molecule FRET data analysis, seeks to lower the threshold for smFRET expertise, allowing for a greater number of scientists to take advantage of this powerful technique.